Full Name
Datukishvili Nelly
Address
8, Abastumani str., Apt.7., Tbilisi 0112, Georgia
Phone
+99532 348981, +995 99 949713
Fax
+99532 939157
E-mail
Web Page
Date Of Birth
1957-05-09
Education
- I. Javakhishvili Tbilisi State University (1974-1979), obtained the skill of physicist (speciality of biophysics). - I. Javakhishvili Tbilisi State University, 1997, obtained the Degree of a candidate of biological sciences (Speciality of Molecular Biology).
Position
Institute of Molecular Biology and Biological Physics – Senior researcher, head of biotechnology group (from September, 2000 – at present).
Publications
- Prangishvili, D., Chinchaladze, D., Bukhrashvili, I., Datukishvili, N., Kutateladze, T., Ketskhoveli,I. (1990) DNA-dependent DNA polymerase of the archaebacterium Sulfolobus acidocaldarius. Abstracts of Bilateral Symposium USSR-FRG, Archaebacteria, Tbilisi, p.37. - Datukishvili, N., Nanazashvili, M., Kvantaliani, Kh., Prangishvili, D. (1992) Characterization of antibodies obtained against DNA polymerase A of archaebacterium Sulfolobus acidocaldarius. Proceedings of the Academy of Sciences of Georgia, Biological Series, vol.18, N4, pp.272-276. - Datukishvili, N., Pokholok, D., Prangishvili, D., Rechinsky, V. (1995) Sulfolobus acidocaldarius DNA polymerase gene, complete cds. GenBank Database, Acc. No.U33846. - Datukishvili, N.,Pokholok, D., Lottspeich, F., Prangishvili, D., Rechinsky, V. (1996) The DNA polymerase- encoding gene from a thermoacidophilic archaeon Sulfolobus acidocaldarius. Gene, 177, pp.271-273. - Datukishvili, N., Pokholok, D., Essipov, D., Prangishvili, D., Rechinsky, V. (1996) Production of a recombinant DNA polymerase from the thermoacidophilic archaeon Sulfolobus acidocaldarius. Abstracts of Bayev memorial conference. Moscow. p.296. - Datukishvili, N., Pokholok, D., Essipov, D., Prangishvili, D., Rechinsky, V. (1996) Production of a recombinant DNA polymerase from the thermoacidophilic archaeon Sulfolobus acidocaldarius. Proceedings of the International Symposium: “The physical-chemical basis of the organization and function of biological systems”, Tbilisi, pp.94-95. - Datukishvili N. (1997) Characterization of the DNA polymerase –encoding gene from the archaeon Sulfolobus acidocaldarius. Proceedings of the Conference of Young Scientists, Tbilisi, pp.5-6. - Datukishvili, N., Tarkhnishvili, G., Mikeladze, D., Sanadze, G. (2000) Investigation of the enzymatic mechanizm of isoprene biosynthesis. Bulletin of the Georgian Academy of Sciences, 162, pp. 523-525. - Datukishvili, N., Tarkhnishvili, G., Mikeladze, D., Beridze, T., Sanadze, G. (2001) Isolation and purification of protein responsible for the conversion of dimethylallylpyrophosphate from Poplar leaves into isoprene. Russian Journal of Plant Physiology (Moscow), 48, pp. 222-225. - Kutateladze,T., Karseladze, M., Zaalishvili, G., Zaalishvili, T., Datukishvili, N. (2005) PCR amplification of DNA from agricultural plants and genetically modified soybean. Bulletin of the Georgian Academy of Sciences, 171, pp. 345-347. - Datukishvili, N., Kutateladze, T., Karseladze, M., Zaalishvili, G., Gabriadze, I., Zaalishvili, T. (2006) Evaluation of DNA extraction methods for PCR analysis of fruit seeds // Proceedings of the Georgian Academy of Sciences, Biological Series, vol.4 (in press). - Datukishvili, N. (2006) Status of Biotechnology in Georgia.// The Joint Meeting of the board of Directors and board of Trustees of the Black Sea Biotechnology Association. //www.bsbanet.org
Conferences,contacts,other scientific and educational activities
- EMBO workshop-95 “Molecular evolution of archaeal, bacterial and organelle genomes”, Aronsborg, Balsta, Sweden. October 6-9, 1995. - Bayev memorial conference. Moscow, 20-22 May, 1996. - International Symposium: “The physical-chemical basis of the organization and function of biological systems”, Tbilisi, 24-27 September, 1996. - Workshop “ Development of biosafety national framework in Georgia”, Tbilisi, 24-25 July, 2003. - Seminar “Risk assessment and management relevant to genetically modified organisms”, Tbilisi, 9-13 March, 2004. - Workshop “Consideration of draft law of Georgia on GMOs”, Tbilisi, 15 April, 2005. - Workshop “INTAS-South-Caucasus 2006”, Tbilisi, 19-21 April, 2006. - International symposium “Biosafety issues in implementation of GMOs: new research approaches, regulation and public perception, Yalta, Crimea, Ukraine, 10-14 May, 2006. - Study tour at Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. During 3 years: 05/1993 – 09/1996. - Study tour at the Agricultural Biotechnology Center, Godollo, Hungary. 6 months, 7/1998 – 12/1998. - Full Member (ID: 20039990350) of Sigma Xi, the Scientific Research Society and of Sigma Xi Georgia.
Participation in Grant Projects
- UNESCO contract SC/RP 206.606.6., 1997-99. - Research fellowship “Detection and characterisation of GMOs in seeds, food, feed, and the environment”, Gent Agricultural Research Centre (DVP-CLO, Belgium), 05.2004-08.2004. - Grants of the Georgian Academy of Sciences.: N14.12, 2000-2001; N14.13.02, 2002-2003; N14.12.04, 2004-2005. - Georgian State scientific programs: 2001-2004; 2005-2008.
Languages
Russian, English
Scientific interests
Molecular biology, Biotechnology, study of genome by DNA-technologies, seed analysis, detection and identification of genetically modified organisms (GMO).
Current scientific activities
In our studies we investigate plant-derived food products by DNA-technologies. We have evaluated different DNA extraction methods, we have developed eucaryot-specific PCR system. The seeds from different plants, in particular: wheat, maize, soybean, barley, oat, French bean, apple, grapevine, lemon, orange, grapefruit and genetically modified (GM) soybean have been analyzed. The quality and quantity of the obtained DNA samples were evaluated by agarose gel electrophoresis, after staining with ethidium bromide. The amplifiability of the extracted DNA samples was estimated by eucaryot- specific PCR using 18S ribosomal RNA gene conserved primer sequences. The suitable DNA extraction methods was selected for each analyte after results interpretation.
Future work plans
Development and optimization of DNA-based PCR methods for detection and identification of genetically modified organisms (GMO).
Department
Computational Sciences